Determination of total phenol content and preliminary phytochemical analysis in Annona reticulata Linn. Leaf extract

Abstract

Plant serves as the main source natural drugs and can be utilized to cure human diseases without much side effects that compared to synthetic medicines. This study is mainly aimed to find out the phytochemicals present in the leaf extract of Annona reticulata Linn. Leaf extract was prepared by three solvents acetone, ethanol and water. Maximum number of phytochemicals presence is reported in ethanolic extract and least number of phytochemicals in aqueous extract. Anthraquinone and protein present only in ethanolic extract. Phenols and flavonoids present in all solvents. Anthraquinones, flavonoid, carbohydrate, proteins, saponins, phenolic group, terpenoids and tannin present in ethanolic extract. In acetone extract, flavonoid, carbohydrate, saponins, phenolic groups and tannins are present. Only saponin and phenolic group obtained from aqueous extract. Quantitative analysis of phenol also calculated in three different solvents. More amount of phenols were observed in ethanolic extract. The amount of phenol that observed in ethanolic leaf extract is 14.05±1.02 mg/g, 3.38 ±0.33 in acetone extract and least amount of phenol in aqueous extract 1.78±1.25 mg/g. phenolic compounds are potent hydrogen donors and they act as an efficient antioxidant.